anti chk1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti chk1
Anti Chk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chk1/pmc13049659-4-0-4
Average 86 stars, based on 1 article reviews

anti chk1 - by Bioz Stars, 2026-10

86/100 stars

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Western Blot:

Article Title: CHK1 is an integral regulator of DNA replication in human cells.
Article Snippet: .. Antibodies AR TI CL E IN P RE SS The following antibodies were used for Western blotting and/or immunostaining: hemagglutinin (HA) tag (Cell Signaling Technology [CST], #2999S); phospho-RPA2 (Ser4/Ser8) (Bethyl Laboratories, A300-245A); phospho-RPA2 (Ser33) (Bethyl Laboratories, A300-246A); RPA2 (CST, #2208S); phospho-CHK1 (Ser296) (CST, #90178S); phospho-CHK1 (Ser317) (CST, #12302S); phospho-CHK1 (Ser345) (CST, #2348S); CHK1 (CST, #2360S); phosphoATM (Ser1981) (CST, #13050S); phospho-histone H2AX (Ser139; γH2AX) (CST, #9718S); histone H2AX (Bethyl Laboratories, A300-082A); phospho-histone H3 (Ser10) (Abcam, ab47297); histone H3 (Abcam, ab8898); cyclin A (Santa Cruz Biotechnology, sc-239); cyclin B (Santa Cruz Biotechnology, sc-245); FLAG tag (Sigma-Aldrich, F3165); and vinculin (SigmaAldrich, V9264). .. The CHK1 inhibitors rabusertib (LY2603618; MedChemExpress, HY-14720) and prexasertib (LY2606368; MedChemExpress, HY-18174), as well as the ATR inhibitor gartisertib (VX-803; MedChemExpress, HY-136270), were obtained from MedChemExpress.

Article Title: CHK1 is an integral regulator of DNA replication in human cells
Article Snippet: .. The following antibodies were used for Western blotting and/or immunostaining: hemagglutinin (HA) tag (Cell Signaling Technology [CST], #2999S); phospho-RPA2 (Ser4/Ser8) (Bethyl Laboratories, A300-245A); phospho-RPA2 (Ser33) (Bethyl Laboratories, A300-246A); RPA2 (CST, #2208S); phospho-CHK1 (Ser296) (CST, #90178S); phospho-CHK1 (Ser317) (CST, #12302S); phospho-CHK1 (Ser345) (CST, #2348S); CHK1 (CST, #2360S); phospho-ATM (Ser1981) (CST, #13050S); phospho-histone H2AX (Ser139; γH2AX) (CST, #9718S); histone H2AX (Bethyl Laboratories, A300-082A); phospho-histone H3 (Ser10) (Abcam, ab47297); histone H3 (Abcam, ab8898); cyclin A (Santa Cruz Biotechnology, sc-239); cyclin B (Santa Cruz Biotechnology, sc-245); FLAG tag (Sigma-Aldrich, F3165); and vinculin (Sigma-Aldrich, V9264). .. The CHK1 inhibitors rabusertib (LY2603618; MedChemExpress, HY-14720) and prexasertib (LY2606368; MedChemExpress, HY-18174), as well as the ATR inhibitor gartisertib (VX-803; MedChemExpress, HY-136270), were obtained from MedChemExpress.

Immunostaining:

FLAG-tag:

Ubiquitin Proteomics:

Article Title: Low dose radiation induces new protein synthesis that promotes cancer radiotherapy resistance.
Article Snippet: Radiotherapy (RT) is a cornerstone of cancer treatment; however, its efficacy is frequently hampered by its adverse effects on normal tissues.. By studying the effects of high-dose radiotherapy (HDRT) and low-dose radiotherapy (LDRT), we found that cancer cells adapt distinct responses to these doses to reduce cytotoxicity.. Upon HDRT, cancThese authors contributed equally to this worker cells initiate a strong DNA damage response (DDR) to gain resistance through rapid production and/or activation of proteins for cell cycle arrest and DNA damage repair.

other:

Article Title: CF10 Displays Improved Synergy with Oxaliplatin in TP53 -Null and Wild-Type CRC Cells from Increased Top1cc and Replication Stress.
Article Snippet: Equal amounts of protein (20–40 μg) were mixed with 2× Laemmli sample buffer containing β-mercaptoethanol, boiled at 95 ◦C for 10 min, separated by SDS-PAGE on a 10% polyacrylamide gel, and transferred to nitrocellulose membranes using a semi-dry BioRad Trans Blot Turbo system (Hercules, CA, USA, RRID: SCR_023156).

Incubation:

Article Title: CF10 Displays Improved Synergy with Oxaliplatin in TP53 -Null and Wild-Type CRC Cells from Increased Top1cc and Replication Stress
Article Snippet: Equal amounts of protein (20–40 μg) were mixed with 2× Laemmli sample buffer containing β-mercaptoethanol, boiled at 95 °C for 10 min, separated by SDS-PAGE on a 10% polyacrylamide gel, and transferred to nitrocellulose membranes using a semi-dry BioRad Trans Blot Turbo system (Hercules, CA, USA, RRID: SCR_023156). .. Nitrocellulose membranes were blocked with 5% non-fat dry milk in TBS-T for 1 h at room temperature, then incubated with primary antibodies against p53 (Cell Signaling, Cat. No. 2524, RRID: AB_331743), p21 (Cell Signaling, Cat. No. 2947, RRID: AB_823586), β-actin (Cell Signaling, Cat. No. 4970, RRID: AB_2223172), Phospho-Chk1 (Ser345) (133D3) (Cell Signaling Technology, Danvers, MA, USA, Cat. No. 2348, RRID: AB_331212), Phospho-Chk2 (Thr68) (Cell Signaling Technology Cat. No. 2197, RRID: AB_2080501), Chk1 (Cell Signaling Technology Cat# 37010, RRID: AB_3662851), Chk2 (Cell Signaling Technology Cat# 6334, RRID: AB_11178526), and Thymidylate Synthase (D5B3) (Cell Signaling Technology Cat# 9045, RRID: AB_2797693) diluted in TBS-T overnight at 4 °C. .. Following primary antibody incubation, membranes were washed three times with TBS-T and incubated with HRP-conjugated secondary (Cell Signaling Technology, Cat# 7074, RRID: AB_2099233) antibodies for 1 h at room temperature.

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